Neutral protease

Neutral protease, a single enzyme preparation for feeding, which is selected as high-yielding strain of Bacillus subtilis(#1398), fermented by advanced deep liquid technology and processed by unique post-treatment technology. It can catalyze the hydrolysis of protein peptide bond into small molecule peptide under neutral or weak acid or weak alkaline conditions, which has good degradation effect on antigenic protein, trypsin inhibitor, lectin, gliadin and other protein anti-nutritional factors. It is beneficial to the absorption and utilization of protein, reducing diarrhea rate, making supplement for the deficiency of animal endogenous enzyme, enhancing appetite, and promoting the growth and development of animals, reducing feed cost, improving the performance of livestock and poultry production.

Function of Protein anti-nutritional factors

Protein anti-nutritional factors in feed mainly includes protease inhibitor, soybean antigen protein (sensitized protein), lectin and gliadin, etc.

They directly or indirectly affect the digestion, absorption and utilization of nutrients in animal intestines in different ways. Even animal health and performance will be affected.(Table 1)

Table 1  Crude protein content in common plant feed materials

Strong sensitization inhibits the activity of digestive enzymes, destroying the normal structure of the small intestine, shielding the absorption of nutrients, reducing the available energy of feed, etc.

〔Neutral protease function〕

 Effective degradation of protein anti-nutritional factors improves animal growth performance and increases weight gaining and feed efficiency.

 Reducing the content of ammonia nitrogen in intestinal digesta. Effectively improving the intestinal morphology. Reducing the injury of intestinal epithelial cells. Reducing feed costs in maintaining the same performance of feed products.

 Reducing the cost of feed in maintaining the performance of unchanged feed products.

 Reducing the difference of different batches of raw materials and stabilize feed quality.

 Improving intestinal microecology, balancing intestinal flora, reducing diarrhea rate and improving disease resistance of animals.

 Reducing fecal nitrogen excretion. Improving barn air quality. Reducing environmental pollution.

〔Neutral protease features〕

 Unique fermented strain

Unique strain, which is R&D together with famous scientific research institute, is  high efficiency.

 Advanced production technology

Deep liquid fermentation technology with unique downstream process; To ensure the high quality and high stability with ISO9001, ISO220000 and EU FAMI-QS quality assurance system and 5S management strandards.

Fig.1  Neutral protease production flat

 Wide range of pH

In line with the physiological pH range of the animal gut, say pH 3.0 to 11.0, the range of action is broad and the duration is longer. (Fig. 2).

Fig. 2 Neutral protease activity changes with pH

 Good temperature resistance

The optimum fermentation strain ensures high temperature resistance of Neutral protease (Fig. 3).

Fig. 3  Storage stability test of neutral protease

 Exogenous addition, improve the growth performance of animals significantly.

Compared with exogenous addition of the two enzymes, the neutral protease significantly increased the daily gain of weaned piglets, the diarrhea rate has decreased significantly.

        Fig. 4  Effects of Two Proteases on whole period growth performance of weaned piglets

〔Product specification and usage〕

 Product specification    Powder：50,000; 100,000U/g；

 Usage

Neutral protease can be used alone or combined with other enzymes  depending on feed composition and purpose. The following are the methods used alone (100,000 U/g product as an example, Table 2) :

Table 2 Application method of neutral protease in full-price compound feed

Note: Powdery enzymes need to be mixed at least twice in the process of feed processing. They can be pre-mixed with vitamins and other trace components first, and then mixed with other feeds.

〔Product selection recommendation〕

 Powder and granulating temperature is less than 85 ℃ of granular material choose powder enzyme; It is recommended to use liquid enzyme after spraying for granular with granulation temperature higher than 85℃.

 If  there is no post-spraying equipment and neutral protease needs to be used in granulating above 85℃. It is suggested to add an additional 20% to 50% safety margin to ensure the full play of product performance.

 Neutral protease can be added to diets of young animals and barley alone.

〔Packing, transportation and storage〕

 Packing: The powder enzyme is packed in 20kg kraft paper bag.

 Transportation: Protected from sun exposure and rain; Shall not be mixed with toxic and harmful and pollutants;

 Storage: Stored in a cool, ventilated and dry place, and the shelf life of powder enzyme is 12 months.

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